The first time that I watched the film, I wasn't aware of how many things in reference to the trans community there were in there. Obviously, I was aware of it going this time in, and boy I wasn't expecting that many. I knew they were there. I didn't know they were basically screaming them throughout the whole film from basically literally every character (even antagonists, though antagonists also play the part of suppressing that).
And I'm pretty sure I didn't even get even remotely close to all of them.
It's much better watching it with this later understanding.